Pre-detection preparations　Syringe Installed Machine repeated freezing and thawing, the cells are destroyed and the intracellular components are released.4), and the cell concentration will reach about 1 million/ml. Collect the supernatant carefully. Urine from a new type of African swine fever tester: collect it in a sterile tube and centrifuge for about 20 minutes (2000-3000 rpm). Quickly freeze and store with liquid nitrogen for later use. Centrifuge for about 20 minutes (2000-3000 rpm).5-10uL, 2-20uL, 20-200uL, 200-1000uL　　 3. Test equipment required for the experiment 1.

High-precision sampler and pipette tip: 0.　　2. Collect the supernatant carefully.　　2. 4. Tissue specimen of the new type of African swine fever tester: After cutting the specimen, weigh it. answer. Add 50;L of stop solution to each well, and measure the OD value of each well at 450nm wavelength with the new type African swine fever tester within 15min. If the test cannot be performed immediately, the specimen can be stored at -20°C, but repeated freezing and thawing should be avoided. Discard the liquid, pat dry on absorbent paper, fill each hole with the washing liquid of the new African swine fever tester, let stand for 1 min

, shake off the washing liquid, pat dry on the absorbent paper, and repeat washing the plate 5 times (can also be washed Plate machine washing plate). If precipitation forms during storage, centrifuge again. 5. Extract as soon as possible after the specimen is collected. Collect the supernatant carefully. Add a certain amount of PBS, pH 7.　　 5.　　 6. Prepare 37℃ constant temperature Box or water bath 4.　　3, need to do 20; washing buffer dilution: distilled water is diluted 1:20, that is, 1 part 20; washing buffer plus 19 parts distilled water.　　3.　

　2. ​​Add 50μL of substrate A and B each to each well, and incubate in a water bath at 37°C, protected from light, for 15 minutes.　　Operation steps of the new type of African swine fever tester　　1.　　7.　　In the use of low-speed centrifuges, how to operate correctly is the key. Next, the prevention and control of the African swine fever epidemic and the restoration of pig production will be the key tasks. Can not detect samples containing NaN3, because NaN3 inhibits horseradish peroxidase (HRP) activity. Centrifuge for about 20 minutes (2000-3000 rpm)

In order to ensure the stability and consistency of drugs, many pharmaceutical companies need to develop from small experiments to pilot trials and then enter the mass production stage in their industrial production.　　 However, according to users, in many pharmaceutical equipment fields, such as spray drying, for many years, many scientific researchers did not have suitable pilot equipment after completing small tests.　　 Generally speaking, the pilot plant needs to consider the process flow, equipment selection, cost control, environmental protection and other issues to make further preparations for subsequent batch equipment.　　

Take the homogenizing emulsifier equipment as an example, six conditions are generally required for the conversion of a small laboratory test to a pilot test. At the same time, in response to the problems of previous production equipment, pharmaceutical equipment companies have gradually transformed traditional production equipment to intelligent production equipment.　　 Of course, in addition to the quality of equipment, Screw Thread Syringe Installed Machine manufacturers also need to pay attention to customer suggestions.

In response to the equipment needs of each stage, pharmaceutical equipment suppliers generally provide corresponding laboratory test, pilot test and industrial production equipment. As the saying goes, 'A good horse is equipped with a good saddle'. Second, the manufacturing conditions in the pilot test have been determined, and the analytical and inspection methods for the product, intermediates, and process principles have been determined; third, the corrosion resistance tests of the pipe materials of some homogeneous emulsification equipment used in the experiment have been carried out, and The necessary complete sets of equipment are available

; fourth, material balance calculations have been carried out, and preliminary treatment methods have been carried out for the three waste problems; fifth, the specifications and unit consumption quantities of raw materials have been proposed; sixth, the requirements for safe production have been proposed

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Release date: April 1, 2016

I went into this expecting young adult. What I got was a graphic novel more inline for a younger reader. My seven year old would have no problem reading this.

This is a compilation of fairy tales; Cinderella, Snow White, Little Red Riding Hood, The Billy Goats Gruff, and Hansel & Gretel. The authors did a great job of making this entertaining and interesting for both boys and girls. Cinderella is a ninja, Ninjarella. What kid won't find that awesome and intriguing?

The graphics are bold and really compliment the dialogue and storyline. They put me in mind of Cartoon Network cartoons.  

I received a pre-release review copy from Netgalley.